科学研究
The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN-β signaling pathway.
编辑:www.4001.com  发布时间:2016-10-21  来源:  阅读次数:
FASEB J.  2016 May;30(5):1757-66. doi: 10.1096/fj.15-281410. Epub 2016 Jan 26.

The VP3 structural protein of foot-and-mouth disease virus inhibits the IFN signaling pathway.

Li D 1,  Yang W 1,  Yang F 1,  Liu H 1,  Zhu Z 1,  Lian K 1,  Lei C 2,  Li S 2,  Liu X 1,  Zheng H 3,  Shu H 4.

Author information

  • 1State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and.
  • 2Collaborative Innovation Center for Viral Immunology, Medical Research Institute, Wuhan University, Wuhan, China.
  • 3State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, National Foot and Mouth Diseases Reference Laboratory, Chinese Academy of Agricultural Sciences, Lanzhou, China; and haixuezheng@163.com.
  • 4Collaborative Innovation Center for Viral Immunology, Medical Research Institute, Wuhan University, Wuhan, China. shuh@whu.edu.cn.

Abstract

Foot-and-mouth disease is a frequently occurring disease of cloven-hoofed animals that is caused by infection with the foot-and-mouth virus (FMDV). FMDV circumvents the type-I IFN response by expressing proteins that antagonize cellular innate immunity, such as leader protease and 3C protease. We identified the FMDV structural protein VP3 as a negative regulator of the virus-triggered IFN signaling pathway. Expression of FMDV VP3 inhibited the Sendai virus-triggered activation of IFN regulatory factor-3 and the expression of retinoic acid-inducible gene-I/melanoma differentiation-associated protein-5. Transient transfection and coimmunoprecipitation confirmed that the structural protein VP3 interacts with virus-induced signaling adapter (VISA), which is dependent on the C-terminal aa 111-220 of VP3. In addition, we found that FMDV VP3 inhibits the expression of VISA by disrupting its mRNA. Taken together, our findings reveal a novel strategy used by the structural VP3 protein of FMDV to evade host innate immunity.-Li, D., Yang, W., Yang, F., Liu, H., Zhu, Z., Lian, K., Lei, C., Li, S., Liu, X., Zheng, H., Shu, H. The VP3 structural protein offoot-and-mouth disease virus inhibits the IFN signaling pathway.

KEYWORDS:

Aphthovirus; innate immunity; pathogen

PMID:  
26813975  
DOI:  
10.1096/fj.15-281410

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