科学研究
The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.
编辑:www.4001.com  发布时间:2016-10-21  来源:  阅读次数:
J Biol Chem.  2016 Jun 17;291(25):13206-15. doi: 10.1074/jbc.M116.718080. Epub 2016 Apr 27.

The Type I Interferon-IRF7 Axis Mediates Transcriptional Expression of Usp25 Gene.

Ren Y 1,  Zhao Y 1,  Lin D 2,  Xu X 2,  Zhu Q 3,  Yao J 1,  Shu HB 4,  Zhong B 5.

Author information

  • 1From the State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China.
  • 2Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China, and.
  • 3State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China.
  • 4Medical Research Institute, School of Medicine, Wuhan University, Wuhan 430071, China.
  • 5From the State Key Laboratory of Virology, College of Life Sciences, Wuhan University, Wuhan 430072, China, Medical Research Institute, School of Medicine, Wuhan University, Wuhan 430071, China, zhongbo@whu.edu.cn.

Abstract

Viral infection or lipopolysaccharide (LPS) treatment induces expression of a large array of genes, the products of which play a critical role in host antipathogen immunity and inflammation. We have previously reported that the expression of ubiquitin-specific protease 25 (USP25) is significantly up-regulated after viral infection or LPS treatment, and this is essential for innate immune signaling. However, the mechanism behind this phenomenon is unclear. In this study, we found that viral infection-induced up-regulation of Usp25 is diminished in cells lacking interferon regulatory factor 7 (IRF7) or interferon α receptor 1 (IFNAR1) but not p65. Sendai virus- or type I interferon-induced up-regulation of Usp25 requires de novo protein synthesis of IRF7. Furthermore, IRF7 directly binds to the two conserved IRF binding sites on the USP25 promoter to drive transcription of Usp25, and mutation of these two sites abolished Sendai virus-induced IRF7-mediated activation of the USP25 promoter. Our study has uncovered a previously unknown mechanism by which viral infection or LPS induces up-regulation of USP25.

KEYWORDS:

USP25; cell signaling; gene regulation; immunology; interferon; interferon regulatory factor (IRF); transcription

PMID:  
27129230  
PMCID:  
PMC4933234
 [Available on 2017-06-17]  
DOI:  
10.1074/jbc.M116.718080

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