YoungNam-Jin
Author:  Date:2019-01-21  Clicks:


 Lab Address: Bldg. #8, Room #1126

                       Medical Research Institute

                       Wuhan University

                       Wuhan, Hubei Province, P.R.China. 430071

 Emal: youngnam_jin@whu.edu.cn

 Phone: +86 13628627281


EDUCATION


2010

Ph.D.

Pharmacology

University of Rochester, Rochester, NY, USA

Advisor: Dr. Gail Johnson-Voll

1999

M.S.

Biochemistry

Seoul National University, Seoul, Korea

Advisor: Dr. Chul-H ak Yang

1997

B.S.

Chemistry

Inha University, Incheon, Korea

  

RESEARCH AND PROFESSIONAL EXPERIENCES

present -

12/2018

Professor

Medical Research Institute, Wuhan University, Wuhan, China

*Research Area: Chemical Biology

*Research Topic: Understand the mechanism of non-canonical translation (PAINT), Dissecting the mechanism of drug’s action, Discovering new small molecules

09/2018 -

05/2018

Assistant Professor (Research)

College of Pharmacy, University of Utah

*Research Area: Zebrafish Chemical Genetics ‘Discovering small molecule modifiers’

*Research Topic: primordial germ cell, RNA regulation, chemical biology, cancer research

04/2018 -

01/2017

Research Associate

College of Pharmacy, University of Utah

Advisor: Dr. Randall Peterson (Dean of College of Pharmacy)

*Research Area: Zebrafish Chemical Genetics ‘Discovering small molecule modifiers’

*Research Topic: primordial germ cell, RNA regulation, chemical biology, cancer research

12/2016 -

01/2011

Research Fellow

Division of Cardiology, Massachusetts General Hospital, Harvard Medical School

Advisor: Dr. Randall Peterson

*Research Area: Zebrafish Chemical Genetics ‘Discovering small molecule modifiers’

*Research Topic: primordial germ cell, RNA regulation

12/2010 - 2007

PhD Student

Department of Pharmacology and Physiology, University of Rochester

*Research Area: Neurodegenerative disease, Molecular and Cell biology

*Research Topic: Study of Protective Effect of PPARgamma on Huntington’s Disease

2007 - 2004

Graduate Assistant

Department of Neurobiology, University of Alabama-Birmingham

*Research Area: Neurodegenerative disease, Transgenic mouse, Electrophysiology

*Research Topic: Role of Usp14 on pathogenesis and synaptic function in ataxia mice

2003 - 2001

Research Assistant

Institute of Neuroscience, Ewha College of Medicine, Seoul, Korea

Supervisor: Dr. Won-Ki Kim

*Research Area: Neuroimmunology, Neuropharmacology

*Research Topic: Effects of the excretory-secretory product of Paragonimus westermani on the nitric oxide production and the cell viability in the primary microglial cell

2001 - 1999

Research Assistant

Struc Biol Center, Korea Institute of Science & Technology, Seoul, Korea

Supervisor: Dr. Yeon Gyu Yu

*Research Area: Biochemistry, Proteomics, Chemical Genomics

*Research Topic: Characterization of Proteins interacting with Doxorubicin using phae displayed method


1999 - 1997

Graduate Student (M.S.)

Department of Chemistry, Seoul National University, Seoul, Korea

*Research Area: Biochemistry, Enzymology

*Research Topic: Identification of an essential tryptophan residue in the activity of alliinase from garlic (Allium sativum) by Chemical Modification


RESEARCH TECHNIQUES

Biochemistry and molecular biology:

genome editing, cloning, protein expression on xenopus oocyte, mutagenesis, cDNA-library construction, phage-related techniques, protein expression and purification, FPLC, HPLC, GC-MS, MALDI-TOF, BIACORE analysis, isothermal titration calorimetry (ITC), spectrophotometric assays

Cell biology:

cell culture (cell lines and primary cultures such as cortical neurons, hippocampal neurons, cerebellar granular neurons, mixed glial cells, astryocytes, and microglial cells), transfection, RNA extraction, RT-PCR, blotting (western, southern, northern, phage), EMSA, immunocytochemistry, immunohistochemistry, immunoprecipitation, cell death/viability assays, luciferase reporter assay, seahorse XF-24 analysis to monitor cell metabolism, viral-mediated expression

Microscope imaging:

epifluorescence microscopy, ratiometric imaging, FRET, confocal microscopy

Electrophysiology:

two electrodes voltage clamp on xenopus oocyte, field recording in brain slices, whole cell patch clamp, cell attached patch clamp

Animal models:

zebrafish, mouse

Surgical skills:

tissue dissection, rat/mice perfusion, stereotactic injection

Computer languages:

Unix, R, Python; Intro level: Perl, Matlab


WORKSHOP ATTENDANCE

04/12/2013 Using CellProfiler for Biological Image Analysis (Broad Institute)

03/06/2013 RNA-Seq Basics (Broad Institute)

12/02/2013 De novo & Genome-guided Transcript Reconstruction from RNA-Seq (Broad Institute)

08/12/2014 GenePattern (Broad Institute)

02/05/2015 Integrative Genomics Analysis with GenomeSpace (Broad Institute)

09/09/2016 FireCloud Workshop (Massachusetts General Hospital)


ADDITIONAL TRAINING CERTIFICATE

Certificate: MATLAB Fundamentals by MathWorks training Services 05/2011

Verified certificate: PH525X: Data Analysis for Genomics by edX HarvardX 07/2014


ACADEMIC AWARDS AND SCHOLARSHIPS

2015-2017

T32 Grant “Cell and Molecular Training for Cardiovascular Biology” 5 T32 HL 7208-38

04/02/2014

Massachusetts General Hospital Scientific Advisory Committee

2014 Poster of Distinction Award

02/02/2014

Keystone Symposia Future of Science Fund scholarship: RNA silencing

2009

Medical Faculty Council (MFC) Travel Award for Basic Sciences

1998

Teaching Assistant

1997

Research Assistant

1994-1996

Merit Scholarship


TEACHING/MENOTORING EXPERIENCE

2017

Mentoring

Qian Xue for Rotational student

Biological Chemistry Program at the University of Utah

2016

Mentoring

Corina Kotidis for Summer internship

Middlesex High School

04/2013 - 04/2012

Mentoring

Andrew P.W. Gonzales for undergraduate thesis

Dept. of Molecular and Cellular Biology, Harvard University

2008 -

2007

Mentoring

for rotational graduate students in Dr. Gail Johnson’s lab

Joshua Allen (2007) from Dept. of Environmental Medicine

Olga Stolpnik (2008) from Dept. of Biomedical Genetics

1998

Teaching Assistant

Dept. of Chemistry, Seoul National University, Seoul, Korea

Instructed general biochemistry and experiments

AFFILIATIONS

2012- present

Genetics Society of America

2013- present

The RNA Society

2003- present

The Society for Neuroscience


PUBLICATIONS

1. Jin YN*§, Schlueter PJ*, Jurisch-Yaksi N, Lam PY, Jin S, Hwang, WY, Yeh JR, Yoshigo M, Ong SE, Schenone M, Hartigan CR, Carr SA, Peterson RT§. (2018) Non-canonical translation via deadenylated 3’UTRs maintains primordial germ cells. Nat Chem Biol. 14(9):844-852. *co-first author, §co-corresponding author (introduced in “News & Views” at the same issue of Nat Chem Biol by Dr. Yuichiro Mishima, Sangyo University, Kyoto, Japan)

2. Metelo AM, Noonan HR, Li X, Jin Y, Baker R, Kamentsky L, Zhang Y, van Rooijen E, Shin J, Carpenter AE, Yeh JR, Peterson RT, Iliopoulos O. (2015) Pharmacological HIF2α inhibition improves VHL disease-associated phenotypes in zebrafish model. J Clin Invest. 125(5):1987-97. PMCID: PMC4463187

3. Nath AK, Ryu JH, Jin YN, Roberts LD, Dejam A, Gerszten RE, Peterson RT. (2015) PTPMT1 Inhibition Lowers Glucose through Succinate Dehydrogenase Phosphorylation. Cell Rep. 10(5): 694-701

4. Jo C, Gundemir S, Pritchard S, Jin YN, Rahman I, Johnson GVW. (2014) Nrf2 reduces levels of phosphorylated tau protein by inducing autophagy adaptor protein NDP52. Nat Commun. 5, 3496

5. Quintanilla RA, Jin YN, von Bernhardi R, Johnson GV. (2013) Mitochondrial permeability transition pore induces mitochondria injury in Huntington disease. Mol Neurodegener. 8, 45. PMCID: PMC3878840

6. Jin YN, Yanxun VY, Gundemir S, Jo C, Cui M, Tieu K, Johnson GVW. (2013) Impaired Mitochondrial Dynamics and Nrf2 Signaling Contribute to Compromised Responses to Oxidative Stress in Striatal Cells Expressing Full-Length Mutant Huntingtin. PLoS One. 8(3):e57932. PMCID: PMC3585875 (recommended by F1000 FACULTY, among the top 10% most cited PLOS ONE articles as of June 2017)

7. Kokel D, Cheung CYJ, Mills R, Coutinho-Budd J, Huang L, Setola V, Sprague J, Jin S, Jin YN, Huang XP, Bruni G, Woolf CJ, Roth BL, Hamblin MR, Zylka MJ, Milan DJ, Randall T Peterson RT. (2013) Photochemical activation of TRPA1 channels in neurons and animals. Nat Chem Biol. 9, 257-263. PMCID: PMC3604056

8. Jin S, Sarkar KS, Jin YN, Liu Y, Kokel D, Van Ham TJ, Roberts LD, Gerszten RE, MacRae CA, Peterson RT..(2013) An In Vivo Zebrafish Screen Identifies Organophosphate Antidotes with Diverse Mechanisms of Action. J Biomol Screen. 18 (1), 108-115

9. Jin YN, Chen PC, Watson JA, Walters BJ, Phillips SE, Green K, Schmidt R, Wilson JA, Johnson GV, Roberson ED, Dobrunz LE, Wilson SM. (2012) Usp14 Deficiency Increases Tau Phosphorylation without Altering Tau Degradation or Causing Tau-Dependent Deficits. PLoS One. 7(10):e47884. PMCID: PMC3483306

10. Jin YN, Hwang WY, Jo C, Johnson GV. (2012) Metabolic State Determines Sensitivity to Cellular Stress in Huntington Disease: Normalization by Activation of PPARγ. PLoS One. 7(1):e30406. PMCID: PMC3262812

11. Quintanilla RA, Dolan PJ, Jin YN, Johnson GV. (2012) Truncated tau and Aβ cooperatively impair mitochondria in primary neurons. Neurobiol Aging. Mar; 33(3):619.e25-35. NIHMSID: NIHMS287090

12. Dolan PJ, Jin YN, Hwang W, Johnson GV. (2011) Decreases in valosin-containing protein result in increased levels of tau phosphorylated at Ser262/356. FEBS Lett. Nov 4;585(21):3424-9. NIHMSID: NIHMS332078

13. Jin YN and Johnson GVW. (2010) The interrelationship between mitochondrial dysfunction and transcriptional dysregulation in Huntington disease. J Bioenerg Biomembr. Jun;42(3):199-205. (review) PMCID: PMC2913874

14. Jin Y, Choi IY, Kim C, Hong S, and Kim WK. (2009) Excretory-secretory products from Paragonimus westermani increase nitric oxide production in microglia in PKC-dependent and -independent manners. Neurosci Res. 65(2): 141-7.

15. Quintanilla RA, Jin YN, Fuenzalida K, Bronfman M, and Johnson GVW. (2008) Rosiglitazone Treatment Prevents Mitochondrial Dysfunction in Mutant Huntingtin-expressing Cells: POSSIBLE ROLE OF PEROXISOME PROLIFERATOR-ACTIVATED RECEPTORg (PPARg) IN THE PATHOGENESIS OF HUNTINGTON DISEASE. J Biol Chem. 283(37): 25628-37. PMCID: PMC2533094

16. Crimmins S, Jin Y, Wheeler C, Huffman AK, Chapman C, Dobrunz LE, Levey A, Roth KA, Wilson JA, and Wilson SM. (2006) Transgenic rescue of ataxia mice with neuronal-specific expression of ubiquitin-specific protease 14. J Neurosci. 26(44):11423-31.

17. Jin Y, Lee JC, Choi IY, Kim EA, Shin MH, and Kim WK. (2006) Excretory-secretory products produced by paragonimus westermani differentially regulate the nitric oxide production and viability of microglial cells. Int Arch Allergy Immunol.139(1):16-24.

18. Kim YK, Lee WK, Jin Y, Lee KJ, Jeon H, and Yu YG. (2006) Doxorubicin Binds to Un-phosphorylated Form of hNopp140 and Reduces Protein Kinase CK2-Dependent Phosphorylation of hNopp140. J Biochem Mol Biol. 39(6):774-81.

19. Lee SJ, Jin Y, Yoon HY, Choi BO, Kim HC, Oh YK, Kim HS, and Kim WK. (2005) Ciclopirox protects mitochondria from hydrogen peroxide toxicity. Br J Pharmacol. 145(4):469-76. PMCID: PMC1576158

20. Kim YK, Jin Y, Vukoti KM, Park JK, Kim EE, Lee KJ, and Yu YG. (2003) Purification and characterization of human nucleolar phosphoprotein 140 expressed in Escherichia coli. Protein Expr Purif. 31(2): 260-264.

21. Choi IY, Jin Y, Nam W, Kim HC, and Kim WK. (2003) Blockade of peroxynitrite-mediated astrocyte death by manganese(III)-cyclam. Neurosci Res. 45(2): 157-61.

22. Jin Y, Yu J, and Yu YG. (2002) Identification of hNopp140 as a Binding Partner for Doxorubicin with a Phage Display Cloning Method. Chem Biol. 9: 157–162.

23. Jin Y, Choi YH, and Yang CH. (2001) Identification of an Essential Tryptophan Residue in Alliinase from Garlic (Allium sativum) by Chemical Modification. Bull Korean Chem Soc. 22(1): 68-76.

ABSTRACTS

1. Jin YN, Schlueter P, Jurisch-Yaksi N, Carr S, and Peterson RT. A novel compound that represses translation via 3’UTR in zebrafish embryos and quiescent cancer cells. Cold Spring Harbor meeting on Eukaryotic mRNA Processing. 08. 19. 2015

2. Jin YN, Schlueter P, Jurisch-Yaksi N, and Peterson RT. A small molecule ablates primordial germ cells through 3’UTR mediated translational repression. Keystone Symposia-RNA Silencing 02. 02. 2014

3. Jin YN, Quintanilla RA, and Johnson GV. Effects of PPARg activation on mitochondrial morphology and function in Huntington’s disease cell model. The Society for Neuroscience/Oct. 13. 2009. (Chicago)

4. Jin YN, Quintanilla RA, and Johnson GV. Protective Effects of PPARg Activation on Mitochondrial Impairment In Striatal Cells Expressing Mutant Huntingtin. The Society for Neuroscience/Nov. 19. 2008. (Washington DC)

5. Kim YK, Jin Y, and Yu YG. Characterization of the Interaction between an Anticancer Drug, Doxorubicin, and Human Nucleolus Phosphoprotein Nopp140; the Biochemical Study about C-terminal of hNopp140. The Korean Society of Molecular and Cellular Biology /Oct. 12. 2001.

6. Jin Y and Yu YG. Identification of a nucleolus protein, hNopp140, as a specific binder to doxorubicin by an affinity selection method with a phage display library. The Pharmaceutical Society of Korea /Apr. 19. 2001.

7. Jin Y and Yu YG. Characterization of Proteins interacting with Doxorubicin using T7 phage displayed Human Liver cDNA library. The Biochemical Society of Korea /Oct. 2000.

8. Jin Y, Choi YH, and Yang CH. The Study of Essential Residues on Alliinase Activity by Chemical Modification and RP-HPLC. The Korean Society for Molecular Biology /Oct. 1998.

9. Jin Y, Choi YH, and Yang CH. The Characterization of the volatile components of Garlic and Ginger by Headspace-Solid Phase Microextraction and GC/MS spectrometry. The Biochemical Society of Korea /Oct. 1997.

10. Choi YH, Jin Y, and Yang CH. Chemical Modification of Alliinase from Garlic; indications for Essential Tyrosyl and Tryptophany Residues at the Active site. The Biochemical Society of Korea /May. 1997.


INVITED TALKS AND ORAL PRESENTATIONS

1. Youngnam N. Jin. When to PAINT & How to PAINT: A non-canonical translation in early zebrafish embryo. Seminar in Department of Pharmacy. University of Utah. 03/20/2017.

2. Youngnam N. Jin. “How do you PAINT?”; Non-canonical translation through deadenylated 3’UTR maintains primordial germ cells. Seminar in Zebrafish Interest Group. University of Utah. 03/20/2017.

3. Youngnam N. Jin. A small molecule translation inhibitor acts via a novel 3'UTR mechanism and ablates primordial germ cells. Department of Systems Biology Retreat. Harvard medical school. 06/01/2016.

4. Youngnam N. Jin. Discovery of an inhibitor of translation via 3’UTR in zebrafish embryos and quiescent cancer cells. Cardiovascular Research Center Retreat. Massachusetts General Hospital, Harvard Medical School. 10/20/2015.

5. Youngnam N. Jin, Peter J. Schlueter, Nathalie Jurisch-Yaksi, Shao-En Ong, Steven Carr, and Randall T. Peterson. The small molecule primordazine ablates primordial germ cells through a novel regulatory element in the nanos1 3’ UTR. Plenary Session II: Signaling and Gene Regulation, 10th International Conference on Zebrafish Development and Genetics. 2012 (Madison).


PATENTS

(1) C-terminal region protein of the human necleolar phosphoprotein hNopp140 that specifically interact with doxorubicin and their gene.

Inventors: Yeon Gyu Yu, Youngnam Jin, Jaehoon Yu.

Application No.: PCT/KR01/01771 (International patent)

Application date: 10/19/2001

Publication Date: 02/13/2003

Publication No.: WO/2003/011904


References

Randall T. Peterson

Professor of Department of Pharmacology & Toxicology

Dean of College of Pharmacy

University of Utah

30 South 2000 East
Salt Lake City, UT 84112

Phone: (801) 581-3402

Email: randall.peterson@pharm.utah.edu


Gail V W Johnson

Professor of Department of Anesthesiology & Perioperative Medicine

Professor of Department of Pharmacology & Physiology

University of Rochester Medical Center
School of Medicine and Dentistry
601 Elmwood Ave, Box 604
Rochester, NY 14642

Phone: (585) 276-3740

Email: gail_johnsonvoll@urmc.rochester.edu


Jing-Ruey Joanna Yeh

Assistant Biologist of Massachusetts General Hospital

Assistant Professor of Harvard Medical School

Massachusetts General Hospital
Cardiovascular Research Center
149 13th Street, 4.217
Charlestown, MA 02129

Phone: (617) 643-3580
Email:
jyeh1@mgh.harvard.edu


Tel: 0086-27-68750205 Fax: 0086-27-68759675  Email: mri@whu.edu.cn

Contact address: Donghu Road, No. 115, Wuchang District, Wuhan, Hubei Province, P.R. China. 430071

Copyright @ 2016 Medical Research Institute

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